Drosphilia Kidney Stones

Our AJP-Renal December podcast focuses on a manuscript describing the use of Drosophila to explore the role of a specific transport protein in the genesis of kidney stones.  Join Dr. Kleyman as he discusses this new model system with Dr. Michael Romero and Dr. Julian Dow.

Romero.jpg Dr. Michael Romero          Dow.jpg Dr. Julian Dow

Taku Hirata, Pablo Cabrero, Donald S. Berkholz, Daniel P. Bondeson, Erik L. Ritman, James R. Thompson, Julian A. T. Dow, and Michael F. Romero. In vivo Drosophilia genetic model for calcium oxalate nephrolithiasis. Am J Physiol Renal Physiol 303: F1555-F1562; published ahead of print September 19, 2012, doi:10.1152/ajprenal.00074.2012.

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In vivo proteolytic activation of γENaC by aldosterone/ Serine protease inhibitors

KenichiroKitamura.jpgOur October 2012 podcast highlights work by Dr. Kenichiro Kitamura and his colleagues at Kumamoto University Graduate School of Medical Sciences. They have recently published two papers in AJP: Renal Physiology: “In vivo contribution of serine proteases to the proteolytic activation of γENaC in aldosterone-infused rats” and “Effect of a serine protease inhibitor on the progression of chronic renal failure.” Join Editor-in-Chief Thomas Kleyman and Dr. Kitamura as they discuss the role of serine proteases in the progressive loss of kidney function in a rat model of CKD, and in the activation of ENaC.

Kohei Uchimura, Yutaka Kakizoe, Tomoaki Onoue, Manabu Hayata, Jun Morinaga, Rika Yamazoe, Miki Ueda, Teruhiko Mizumoto, Masataka Adachi, Taku Miyoshi, Naoki Shiraishi, Yoshiki Sakai, Kimio Tomita, and Kenichiro Kitamura. In vivo contribution of serine proteases to the proteolytic activation of γENaC in aldosterone-infused rats Am J Physiol Renal Physiol October 1, 2012 303:F939-F943; published ahead of print July 25, 2012, doi:10.1152/ajprenal.00705.2011

Manabu Hayata, Yutaka Kakizoe, Kohei Uchimura, Jun Morinaga, Rika Yamazoe, Teruhiko Mizumoto, Tomoaki Onoue, Miki Ueda, Naoki Shiraishi, Masataka Adachi, Taku Miyoshi, Yoshiki Sakai, Kimio Tomita, and Kenichiro Kitamura.Effect of a serine protease inhibitor on the progression of chronic renal failure Am J Physiol Renal Physiol; published ahead of print July 25, 2012, doi:10.1152/ajprenal.00706.2011

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KS-WNK1 in cortical thick ascending limb

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Our September 2012 podcast has AJP-Renal associate editor, Lisa Satlin and Dr. Chou-Long Haung of UT Southwestern discussing his recent paper which studied the effect of KS-WNK1 on Na transport in the cortical thick ascending limb and its role in the regulation of sodium transport by dietary K.

Chih-Jen Cheng, Thao Truong, Michel Baum, and Chou-Long Huang. Kidney-Specific WNK1 Inhibits Sodium Reabsorption in Cortical Thick Ascending Limb. Am J Physiol Renal Physiol; published ahead of print July 11, 2012, doi:10.1152/ajprenal.00290.2012

Flow induced regulation of PGE2

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For August we are highlighting a paper by Raheev Rohatgi and co-workers entitled “Flow induced prostaglandin E2 release regulates Na and K transport in the collecting duct”.  Listen along as Dr. Rohatgi and Editor-in-Chief Tom Kleyman discuss the role of tubular flow in activating phospholipase A2 and excretion of PGE2, and the role of PGE2 in regulating ion transport in collecting ducts.

Daniel Flores, Yu Liu, Wen Liu, Lisa M. Satlin, and Rajeev Rohatgi. Flow induced prostaglandin E2 release regulates Na and K transport in the collecting duct. Am J Physiol Renal Physiol; published ahead of print June 13, 2012, doi:10.1152/ajprenal.00169.2012

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ENaC inhibition stimulates Cl- secretion in mouse CCD

Pech001.jpgOur July 2012 podcast highlights a paper published by Dr. Valdimir Pech and his colleagues entitled “ENaC inhibition stimulates Cl- secretion in the mouse cortical collecting duct through an NKCC1-dependent mechanism”. Join us as Editor-in-Chief Thomas Kleyman and Vladimir Pech discuss  the role of electrogenic Na+ absorption in Cl- absorption, and how Dr. Pech's work contrasts with previous studies addressing Cl- absorption in the CCD.

Vladimir Pech, Monika Thumova, Young Hee Kim, Diana Agazatian, Edith Hummler, Bernard C. Rossier, Alan M. Weinstein, Masayoshi Nanami, and Susan M. Wall. ENaC inhibition stimulates Cl secretion in the mouse cortical collecting duct through an NKCC1-dependent mechanisn. Am J Physiol Renal Physiol July 1, 2012 303:F45-F55; published ahead of print April 11, 2012, doi:10.1152/ajprenal.00030.2012

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RAS in Glomerular Endothelial Cells

bellpd.jpgvelezjphoto.jpgFor our June Podcast, we  join associate editor Darwin Bell as he speaks with Juan Carlos Velez of The Medical University of South Carolina about his recently published paper, “Enzymatic Processing of Angiotensin Peptides by Human Glomerular Endothelial Cells”. Listen in as they discuss the processing of angiotensin by glomerular endothelial cells.

Juan Carlos Q Velez, Jessalyn L Ierardi, Alison M Bland, Thomas A. Morinelli, John M Arthur, John R. Raymond, and Michael G. Janech. Enzymatic Processing of Angiotensin Peptides by Human Glomerular Endothelial Cells . Am J Physiol Renal Physiol; published ahead of print March 28, 2012, doi:10.1152/ajprenal.00087.2012

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PKCα and Urea Transport

In our newest podcast, Editor in Chief Thomas Kleyman interviews senior author and past Editor in Chief Jeff Sands along with lead author Janet Klein (Emory University) about the dual phosphorylation pathways that regulate urea transporter activity in the kidney inner medulla.

Janet D. Klein, Christopher F. Martin, Kimilia J. Kent, and Jeff M. Sands. Protein kinase C-α mediates hypertonicity-stimulated increase in urea transporter phosphorylation in the inner medullary collecting duct. Am J Physiol Renal Physiol May 1, 2012 302:F1098-F1103; published ahead of print February 1, 2012, doi:10.1152/ajprenal.00664.2011

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NHE3 regulates calcium homeostasis

Alexander.jpgListen as Associate Editor Susan Wall and Dr. Todd Alexander discuss mechanisms of calcium absorption in the nephron and Dr. Alexander's recent publication in AJP-Renal, “THE EPITHELIAL SODIUM-PROTON EXCHANGER, NHE3, IS NECESSARY FOR RENAL AND INTESTINAL CALCIUM (RE)ABSORPTION.”

Wanling Pan,Jelena Borovac,Zachary Spicer,Joost G. Hoenderop,René J. Bindels,Gary E. Shull, Michael R. Doschak, Emmanuelle Cordat, and R. Todd Alexander.The epithelial sodium/proton exchanger, NHE3, is necessary for renal and intestinal calcium (re)absorption. Am J Physiol Renal Physiol April 15, 2012 302:F943-F956; published ahead of print September 21, 2011, doi:10.1152/ajprenal.00504.2010

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Regulation of the Pendrin Gene by Uroguanylin

iz.jpgIn our second Podcast of March, the manuscript “The Pendrin Anion Exchanger Gene is Transcriptionally Regulated by Uroguanylin: A Novel Entero-Renal Link” is discussed by Associate Editor Susan Wall and Prof. Israel Zelikovic. Listen in as they speak on a novel enterorenal link  -  transcriptional regulation of the pendrin anion exchanger gene by uroguanylin via a heat shock system-dependent mechanism.

Julia Rozenfeld, Osnat Tal, Orly Kladnitsky, Lior Adler, Edna Efrati, Stephen L. Carrithers, Seth L. Alper, and Israel Zelikovic. The pendrin anion exchanger gene is transcriptionally regulated by uroguanylin: a novel enterorenal link. Am J Physiol Renal Physiol March 1, 2012 302:F614-F624; published ahead of print November 30, 2011, doi:10.1152/ajprenal.00189.2011

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Unanswered Questions- Autoregulation

This is the first podcast by AJP:Renal addressing the important unanswered questions remaining in renal physiology. The purpose of this series is to give students, postdocs and junior faculty access to the ideas of leaders in their field. In this podcast William Arendshorst and Christopher Wilcox will discuss the big questions remaining to be solved in the field of autoregulation of renal blood flow. They address the mechanisms involved and how they are altered in pathological states.

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